enterokinase ek (New England Biolabs)
Structured Review
Enterokinase Ek, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enterokinase+ek/Enterokinase%2C+light+chain/pm41794036-642-7-9
Average 96 stars, based on 292 article reviews
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other:Article Title: Glycopeptide probes of PSGL-1 to study site-specific glycosylation and stem cell homing function Article Snippet: Half of the protein was digested with 0.03 pg/ml Article Title: Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1 Article Snippet: Half of the protein was digested with 0.03 μg/ml Article Title: Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1 Article Snippet: Half of the protein was digested with 0.03 g/ml Article Title: Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1 Article Snippet: His-tagged protein was then captured onto a HisTrap HP column using an AKTA FPLC system (GE Healthcare), the column was washed with sodium phosphate buffer (20 m m sodium phosphate, 500 m m NaCl, pH 7.4) containing 25–100 m m imidazole, and the bound protein was eluted using sodium phosphate buffer containing 200 m m imidazole. Article Title: Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1 Article Snippet: The eluted sample was reacted with 200 μ m dibenzylcyclooctyne (DBCO)-PEG4-biotin conjugate (Click Chemistry Tools, Scottsdale, AZ) for 3 h at room temperature. Variant Assay:Article Title: Cytoplasmic tail diversity determines the effector bias of the adhesion GPCR ADGRL2. Article Snippet: Article Cytoplasmic tail diversity determines the effector bias of the adhesion GPCR ADGRL2 Cloning:Article Title: The family 1 glycoside hydrolase from Clostridium cellulolyticum H10 is a cellodextrin glucohydrolase. Article Snippet: The only family 1 glycoside hydrolase in Clostridium cellulolyticum H10 (CcGH1) is annotated as a beta-galactosidase but has high sequence homology with many betaglucosidases.. Given the possible importance of beta-glucosidase in cellulose utilization by C. cellulolyticum, the encoding open reading frame Ccel_0374 was cloned and expressed in E. coli as a soluble fusion protein with thioredoxin.. After tag cleavage, the purified enzyme had a molecular mass of 52 kDa and was active in dimeric form on a broad range of substrates, including cellobiose, cellotriose, cellotetraose, p-nitrophenyl-beta-glucopyranoside, lactose, and o-nitrophenyl-beta-galactopyranoside. |
