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enterokinase ek  (New England Biolabs)


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    Structured Review

    New England Biolabs enterokinase ek
    Enterokinase Ek, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enterokinase+ek/Enterokinase%2C+light+chain/pm41794036-642-7-9
    Average 96 stars, based on 292 article reviews
    enterokinase ek - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Glycopeptide probes of PSGL-1 to study site-specific glycosylation and stem cell homing function
    Article Snippet: Half of the protein was digested with 0.03 pg/ml enterokinase (EK) (New England Biolabs, Ipswich, MA) overnight at RT in 20 mM Tris—HCI, 200 mM NaCl and 2 mM CaClz, pH 7.2.

    Article Title: Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1
    Article Snippet: Half of the protein was digested with 0.03 μg/ml enterokinase (EK) (New England Biolabs, Ipswich, MA) overnight at room temperature in 20 m m Tris-HCl, 200 m m NaCl, and 2 m m CaCl 2 , pH 7.2.

    Article Title: Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1
    Article Snippet: Half of the protein was digested with 0.03 g/ml enterokinase (EK) (New England Biolabs, Ipswich, MA) overnight at room temperature in 20 mM Tris-HCl, 200 mM NaCl, and 2 mM CaCl2, pH 7.2.

    Article Title: Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1
    Article Snippet: His-tagged protein was then captured onto a HisTrap HP column using an AKTA FPLC system (GE Healthcare), the column was washed with sodium phosphate buffer (20 m m sodium phosphate, 500 m m NaCl, pH 7.4) containing 25–100 m m imidazole, and the bound protein was eluted using sodium phosphate buffer containing 200 m m imidazole.

    Article Title: Competition between Core-2 GlcNAc-transferase and ST6GalNAc-transferase Regulates the Synthesis of the Leukocyte Selectin Ligand on Human P-selectin Glycoprotein Ligand-1
    Article Snippet: The eluted sample was reacted with 200 μ m dibenzylcyclooctyne (DBCO)-PEG4-biotin conjugate (Click Chemistry Tools, Scottsdale, AZ) for 3 h at room temperature.

    Variant Assay:

    Article Title: Cytoplasmic tail diversity determines the effector bias of the adhesion GPCR ADGRL2.
    Article Snippet: Article Cytoplasmic tail diversity determines the effector bias of the adhesion GPCR ADGRL2

    Cloning:

    Article Title: The family 1 glycoside hydrolase from Clostridium cellulolyticum H10 is a cellodextrin glucohydrolase.
    Article Snippet: The only family 1 glycoside hydrolase in Clostridium cellulolyticum H10 (CcGH1) is annotated as a beta-galactosidase but has high sequence homology with many betaglucosidases.. Given the possible importance of beta-glucosidase in cellulose utilization by C. cellulolyticum, the encoding open reading frame Ccel_0374 was cloned and expressed in E. coli as a soluble fusion protein with thioredoxin.. After tag cleavage, the purified enzyme had a molecular mass of 52 kDa and was active in dimeric form on a broad range of substrates, including cellobiose, cellotriose, cellotetraose, p-nitrophenyl-beta-glucopyranoside, lactose, and o-nitrophenyl-beta-galactopyranoside.



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    GenScript corporation his-tagged bovine enterokinase (ek)
    GrB mut -TRA construct design and physical characterization. A Schematic representation of DNA vectors required for expressing Pro-GrB mut -TRA as a human IgG1 antibody in Expi293 cells. Following affinity chromatography purification, EK cleavage was required to generate a catalytically active GrB mut -TRA molecule. B SDS-PAGE and Coomassie Blue staining of purified TRA and GrB mut -TRA under reducing and non-reducing conditions. Arrow insets indicate HC and LC bands for each molecule. C Western blot analysis of TRA, Pro-GrB mut -TRA, and GrB mut -TRA using reagents specific to human IgG and GrB. Abbreviations used: EK, <t>enterokinase;</t> GrB, granzyme B; rGrB, recombinant GrB; GrB mut -TRA, catalytically active AFP; HC, heavy chain; LC, light chain; NR, non-reduced; Pro-GrB mut -TRA, catalytically inert molecule; R, reduced; TRA, trastuzumab
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    GrB mut -TRA construct design and physical characterization. A Schematic representation of DNA vectors required for expressing Pro-GrB mut -TRA as a human IgG1 antibody in Expi293 cells. Following affinity chromatography purification, EK cleavage was required to generate a catalytically active GrB mut -TRA molecule. B SDS-PAGE and Coomassie Blue staining of purified TRA and GrB mut -TRA under reducing and non-reducing conditions. Arrow insets indicate HC and LC bands for each molecule. C Western blot analysis of TRA, Pro-GrB mut -TRA, and GrB mut -TRA using reagents specific to human IgG and GrB. Abbreviations used: EK, enterokinase; GrB, granzyme B; rGrB, recombinant GrB; GrB mut -TRA, catalytically active AFP; HC, heavy chain; LC, light chain; NR, non-reduced; Pro-GrB mut -TRA, catalytically inert molecule; R, reduced; TRA, trastuzumab

    Journal: BMC Biology

    Article Title: Modeling antibody drug conjugate potential using a granzyme B antibody fusion protein

    doi: 10.1186/s12915-024-01860-x

    Figure Lengend Snippet: GrB mut -TRA construct design and physical characterization. A Schematic representation of DNA vectors required for expressing Pro-GrB mut -TRA as a human IgG1 antibody in Expi293 cells. Following affinity chromatography purification, EK cleavage was required to generate a catalytically active GrB mut -TRA molecule. B SDS-PAGE and Coomassie Blue staining of purified TRA and GrB mut -TRA under reducing and non-reducing conditions. Arrow insets indicate HC and LC bands for each molecule. C Western blot analysis of TRA, Pro-GrB mut -TRA, and GrB mut -TRA using reagents specific to human IgG and GrB. Abbreviations used: EK, enterokinase; GrB, granzyme B; rGrB, recombinant GrB; GrB mut -TRA, catalytically active AFP; HC, heavy chain; LC, light chain; NR, non-reduced; Pro-GrB mut -TRA, catalytically inert molecule; R, reduced; TRA, trastuzumab

    Article Snippet: Eluted Pro-Grb mut -TRA IgG antibody was desalted in PBS using PD-10 columns (Cytiva) and enzymatically digested for 48 h at room temperature (RT) with His-tagged bovine enterokinase (EK) (GenScript) following the manufacturer’s instructions.

    Techniques: Construct, Expressing, Affinity Chromatography, Purification, SDS Page, Staining, Western Blot, Recombinant